硕士研究生《检验医学进展》课程将于下周继续上课，具体安排如下：

吴广

 Dr.Wu is an Assistant Research Fellow at the Medical Science ResearchCenter of Hallym University, Korea. He received his Ph.D. degree from the Collegeof Medicine, Hallym University in 2011. From 2012-2015, he underwentpostdoctoral fellowship training at Medical Science Research Center of HallymUniversity. His research focuses on the production of monoclonal antibodies againsttumor-associated antigens and their functions.

讲座简介

  Transmembrane 4 superfamily member 5 protein (TM4SF5) isa potential therapeutic target for hepatocellular carcinoma (HCC) and coloncancer. We designed a cyclic peptide targeting TM4SF5. Cyclic peptide-specificantibodies were produced in mice after immunization with a complex of thepeptide, CpG-DNA, and liposomes. Intravenous injection of the CT-26 mouse coloncancer cell line into mice induced tumors in the lung. Immunization with thepeptide vaccine improved the survival rate and reduced the growth of lungtumors. We established a monoclonal antibody specific to the cyclicTM4SF5-based peptide and humanized the antibody sequence by complementaritydetermining region-grafting. The humanized antibody was reactive to the cyclicpeptide and TM4SF5 protein. Treatment of CT-26 cells with the humanizedantibody reduced cell motility in vitro. Furthermore, direct injection of thehumanized anti-TM4SF5 antibody in vivo reduced growth of lung tumors in mousemetastasis model. Therefore, we conclude that the immunization with the cyclicpeptide vaccine and injection of the TM4SF5-specifc humanized antibody have ananti-metastatic effect against colon cancer in mice. Importantly, the humanizedantibody may serve as a starting platform for further development andapplication in clinical settings.